Simple procedure for preparing trifluoroacetylprolyl amino acids, acyl components in the racemization test for peptide synthesis by GLC.
نویسندگان
چکیده
Amongthe methods for racemization testing in peptide synthesis, the GLCmethod is a simple one and the most practicable, especially when a packed column is used. In this method Tfa-Pro-X-OH (X=amino acids) is coupled with H-Val-Pro-OMe, the resulting Tfa-tetrapeptide-OMe is separated by GLC, and the degree of recemization is estimated from the ratio of the peaks separated to the diastereoisomers ldll and llll.1 2) The Tfa-Pro-X-OH used in this method has been prepared in several ways:
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عنوان ژورنال:
- Agricultural and biological chemistry
دوره 54 5 شماره
صفحات -
تاریخ انتشار 1990